| The NeuroCult® NCFC Assay Kit can be used to: | | – | • | Quantify neural stem and progenitor cells based on proliferative potential (colony size) | | | | | | – | • | Compare neural stem and progenitor cell content in diverse cell samples, such as primary embryonic or adult cells, or cells from cultured neurospheres | | | | | | | • | Study effects of cytokines or other compounds on stem cell regulation | | | | | | – | • | Identify changes in neural stem and progenitor cell numbers in transgenic or other animal models by comparing colony numbers between transgenic and non-transgenic animals |
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Louis et al. recently published an article in Stem Cells, reporting the development and validation of the NCFC Assay.
ORDERING INFORMATION | Product | Quantity | Catalog # | | NCFC Assay Kit (Mouse) | 1 kit | 05740 | | NCFC Assay Kit (Rat) | 1 kit | 05742 |
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| | How does the assay work? | | Primary or cultured neural cells are grown in a semi-solid collagen-based medium using the NeuroCult Neural Colony-Forming Cell (NCFC) Assay Kit. Neural stem and progenitor cells have different proliferative potentials and produce colonies of different sizes. At the end of the culture period, clonally-derived colonies of different sizes are counted. Neural stem cells proliferate to form the largest colonies (>2 mm in diameter) while progenitor cells have limited proliferative potential and form smaller colonies. |
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